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Arq. biol. tecnol ; 31(2): 313-20, 1988. tab
Article in English | LILACS | ID: lil-69596

ABSTRACT

Poly (2'-O-methyladenylic acid), poly (Am), is recognized as template in the reaction catalyzed by avian myeloblastosis virus, AMV, reverse transcriptase better in the presence of Mn than Mg as divalent cation. An apparent KM value of 2.5 x 10 M in relation to TTP and an inhibition of the reaction at poly (Am concentration higher than 5 microng/ml have been observed. Ethidium bromide, EtBr, tetramethyl ethidium bromide, TMEtBr, dideoxy TTP, and novobiocin are inhibitors of the poly (Am)-directed reverse transcriptase reaction in the presence of Mn


Subject(s)
Poly A/metabolism , RNA-Directed DNA Polymerase/metabolism , Templates, Genetic , DNA-Directed DNA Polymerase/biosynthesis , Avian Myeloblastosis Virus/enzymology
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